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KMID : 0363320070280020230
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Journal of Korean Oriental Internal Medicine
2007 Volume.28 No. 2 p.230 ~ p.241
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Protective Effects of Phenolic-rich Fraction(PRF) from Fructus Schisandrae on H_2O_2-induced Apoptosis of SH-SY5Y Cells
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Son In-Hwan
Lee Key-Sang
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Abstract
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Objective : This study was intended to ascertain the protective effect of phenolic-rich fraction (PRF) from Fructus Schisandrae on SH-SY5Y cells.
Methods : PRF was obtained from the 80% ethanol extract of Fructus Schisandrae by Sepabeads SP-850 column chromatography. The neuroprotective effect of the FS PRS was investigated due to the hydrogen peroxide (H_2O_2)-induced apoptosis of cultured SH-SY5Y cells.
Results : Cell viability assays revealed that pretreating SH-SY5Y cells with PRF (10-200 {mu}g/mL) resulted in significant dose-dependent protection against H_2O_2-induced cell death. The effect was assessed by flow cytometric analysis of DNA contents using propidium iodide (PI) staining. The population of apoptotic cells was increased by 32.89% in only H_2O_2 (150 {mu}M)-treated environment, but it was reduced by pre-treatment of FS PRF (200 {mu}g/mL) to 21.61%. H_2O_2-induced caspase-3 activation and PARP cleavage were reduced in FS PRF pre-treated cells, and PRF led to an apparent suppressive effect on the oxidative stress induced by reactive oxygen species (ROS).
Conculsion : This study showed that Fructus Schisandrae should be useful for the treatment prevention of neurodegenerative diseases associated with elevated ROS levels.
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KEYWORD
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Fructus Schisandrae, PRF, SH-SY5Y, H_2O_2, Apoptosis
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